Molecular Identification of Multi Drug-resistant Klebsiella pneumoniae Isolated from Neonates with Sepsis in Egypt

Neonatal sepsis is one of the most prevalent infections today, claiming lives almost million newborns in developing countries. Our objective to determine bacteria that cause Egyptian hospitals, as well discover their resistance profiles, allowing for selection appropriate drug combinations against multidrug resistant (MDR) bacteria. Nine hundred and eighty-nine blood samples were collected from neonates Al Demerdash Ain Shams University Specialized Hospital March 2019 20121 according standard protocols. Bacterial identification antimicrobial susceptibility test AST performed using VITEK® 2 system. The results revealed 51.4 % patients had positive cultures, with 60.3 females 39.7 %. Males. Sepsis late onset was detected 95% cases, while early observed only 5% total cases. According study, 98.3 Klebsiella Pneumoniae isolates Ampicillin, 91% Ampicillin/sulbactam, 90% Ceftazidime Cefotaxime. Quantitative Real-time RT-PCR analysis blaCTX-M-15 gene expression found (69%) MDR K. pneumoniae expressed at a significantly higher level than susceptible isolates, mean fold change 10.0349 versus 1.8646. In conclusion, leading neonatal majority hospitals' NICUs. Application infection prevention control strategies, conjunction stewardship programs, may be beneficial overcoming this challenge.